Abstract     Objective: The objective of the present study was to ascertain the prevalence of Uropathogens and to study the antimicrobial resistance among them with commonly used antibiotics retrospectively. Methods: The urine specimens were collected from patients with symptoms of UTI from Govt. Villlupuram medical college hospital were cultured by standard microbiological procedures for the isolation of uropathogens and the isolates were subjected to antimicrobial susceptibility assay to investigate the resistance pattern of isolates. Results: A total of 1817 non-duplicative urine specimens were collected, of which 478 (26.3%) were found to be positive for urine culture. The positive samples were from both 223 (46.65%) male and 255 (53.35%) female patients. In the present study 491 isolates were obtained from the culture positive urine (n=478) specimens, of which 445/491 (90.6%) were found to be Gram negative bacilli and 66/491 (13.4%) were found to be Gram positive cocci. Conclusion: Isolates form urine showed high degree of resistance against the panel of antibiotics used to determine the antibacterial susceptibility.

Keywords: UTI, urine culture, drug resistance, antibiotic susceptibility.