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International Journal of Recent Trends in Science and Technology, ISSN 2277-2812 E-ISSN: 2249-8109

Volume 12, Issue 2, September 2014 pp 262-265

Research Article

A comparative study of platelet counts by manual and automated methods in platelet poor plasma

J B Leena^1*, Mrudula², Sumanth Devaraju³, Crysle Saldhana¹, Veronica⁴

¹Assistant Professor,²B Sc MLT Student, ³Associate Professor,⁴MBBS Student, Department of Pathology,^, Father Muller Medical College, Kankanady Road, Kankanady, Manglore 575002 – Karnataka, INDIA.

Abstract

Introduction: Reliable platelet count is crucial for minimizing errors in coagulation studies. Platelet poor plasma is routinely used for coagulation studies as platelets interfere with coagulation. In this study randomly collected 30 samples of platelet poor plasma for routine coagulation studies received in haematology laboratory of Father Muller Medical College Hospital, Mangalore for a period of 3 months were analysed and counts were done by both manual method and automated methods. Platelets were analyzed by using Neubauer chamber and were compared with automated method. The study analysis showed that there was no significant difference between manual and automated platelet counts in platelet poor plasma. The intraclass correlation between automated and manual method showed significance with p value 0.00, and also showed an excellent agreement (ICC: 0.868) with a positive correlation between the two methods. Hence this study shows that both methods can be used to asses platelets in platelet poor plasma as a part of quality control procedure. Manual method is thus recommended even at places where automation is unavailable to improve quality of coagulation studies.