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International Journal of Recent Trends in Science and Technology, E-ISSN: 2249-8109

 Volume 1, Issue 2, 2011 pp 56-60

Date of Publish: 08 November 2011

Original Article

ANTIBIOGRAM OF GRAM NEGATIVE UROPATHOGENS IN HOSPITALIZED PATIENTS

K.D. Deshpande¹, A.P. Pichare², N.M. Suryawanshi³, M.S. Davane⁴

^1,3,4 Resident, Department of Microbiology, MIMSR Medical College, Latur(MS),INDIA.

²Professor & HOD Department of Microbiology, MIMSR Medical College, Latur (MS),INDIA.

Academic Editor:  Dr. Pawar D.D.

Background: Urinary tract infections (UTIs) are caused by variety of microorganisms. The frequency of different bacterial isolates and their susceptibility to antibiotics may differ widely, particularly in hospitalized patients. The study of susceptibility pattern becomes obligatory for a proper selection of antibiotics.

Aim: To evaluate antimicrobial susceptibility pattern of the Gram negative organisms isolated from urine cultures in hospitalized patients. Material and Methods: The present study was conducted in Yashwantrao Chavan Rural Hospital, Latur over a period of one year from June 2010 to May 2011. A total of 500 urine samples from hospitalized patients which showed significant bacteriuria were studied. Samples were inoculated on Blood agar and MacConckey agar. Further identification of organisms was done by standard Microbiological methods. Antimicrobial Susceptibility pattern was studied by Modified Kirby-Bauer�s disc diffusion method with the panel of 15 drugs as per Clinical Laboratories Standard Institute (CLSI) guidelines.

Results: UTIs were found more common in females 296 (59.2%). Commonest organism found was Escherichia coli 252 (50.2%) followed by Klebsiella spp. 123 (24.6%), Pseudomonas spp. 49 (9.8%), Proteus spp. 42 (8.4%), Citrobacter spp. 27(5.4%) and Acinetobacter spp. 8 (1.6%). Majority of the strains were found sensitive to nitrofurantoin 404 (80.8%) followed by amikacin 357 (71.4%), piperacillin-tazobactam 325 (65.2%) and co-trimoxazole 254 (50.8%). Commonly prescribed fluroquinolones were found least effective for treatment of UTI. All the strains were found sensitive to imipenem. Extended spectrum beta lactamase (ESBL) was noted in 169 (67.33%) E.coli and 87 (70.73%) in Klebsiella spp.

Conclusions: To discourage the indiscriminate use of antibiotics and to prevent further development of bacterial drug resistance, proper knowledge of susceptibility pattern of uropathogens in particular area is very important before prescribing empirical antibiotic therapy.