Deshpande1, A.P. Pichare2, N.M. Suryawanshi3,
Resident, Department of Microbiology, MIMSR Medical College,
& HOD Department of Microbiology, MIMSR Medical College, Latur (MS),INDIA.
Academic Editor: Dr.
Urinary tract infections (UTIs) are caused by variety of microorganisms.
The frequency of different bacterial isolates and their susceptibility
to antibiotics may differ widely, particularly in hospitalized patients.
The study of susceptibility pattern becomes obligatory for a proper
selection of antibiotics.
To evaluate antimicrobial susceptibility pattern of the Gram negative
organisms isolated from urine cultures in hospitalized patients.
Material and Methods: The present study was conducted in Yashwantrao
Chavan Rural Hospital, Latur over a period of one year from June 2010 to
May 2011. A total of 500 urine samples from hospitalized patients which
showed significant bacteriuria were studied. Samples were inoculated on
Blood agar and MacConckey agar. Further identification of organisms was
done by standard Microbiological methods. Antimicrobial Susceptibility
pattern was studied by Modified Kirby-Bauer’s disc diffusion method with
the panel of 15 drugs as per Clinical Laboratories Standard Institute (CLSI)
UTIs were found more common in females 296 (59.2%). Commonest organism
found was Escherichia coli 252 (50.2%) followed by Klebsiella spp. 123
(24.6%), Pseudomonas spp. 49 (9.8%), Proteus spp. 42 (8.4%), Citrobacter
spp. 27(5.4%) and Acinetobacter spp. 8 (1.6%). Majority of the strains
were found sensitive to nitrofurantoin 404 (80.8%) followed by amikacin
357 (71.4%), piperacillin-tazobactam 325 (65.2%) and co-trimoxazole 254
(50.8%). Commonly prescribed fluroquinolones were found least effective
for treatment of UTI. All the strains were found sensitive to imipenem.
Extended spectrum beta lactamase (ESBL) was noted in 169 (67.33%) E.coli
and 87 (70.73%) in Klebsiella spp.
To discourage the indiscriminate use of antibiotics and to prevent
further development of bacterial drug resistance, proper knowledge of
susceptibility pattern of uropathogens in particular area is very
important before prescribing empirical antibiotic therapy.