Antifungal Properties of
Gamma-Irradiated Chitosan from Sea Crab Shells
A. Adu-Gyamfi1, E. A. Quarcoo1, Y. Serfor-Armah2,
Biotechnology and Nuclear Agriculture Research Institute, Ghana Atomic
Energy Commission, P.O. Box LG 80, Legon, GHANA.
National Nuclear Research Institute, Ghana Atomic Energy Commission,
P.O. Box LG 80, Legon, GHANA.
Academic Editor: Dr.
Aher K. R.
Chitosan extracted from sea crab shells
was used to determine antifungal properties against Aspergillus niger.
Chitosan powder irradiated at 100 kGy and dissolved in 1 % acetic acid
(v/v) with pH adjusted to approximately 6.0 was used in preparing
chitosan concentrations of 2 %, 1.5 %, 1 % and 0.5 %. The agar dilution
method was used to test the antifungal activity of the various chitosan
solutions at concentrations of 0.20 %, 0.15 %, 0.10 % and 0.05 %. Both
media containing irradiated and unirradiated chitosan inhibited the
mycelial growth of Aspergillus niger and the degree of inhibition
was dependent on the concentration of the chitosan in the fungal growth
medium. Results show that the media containing irradiated chitosan
inhibited the mycelia growth of Aspergillus niger to a greater
extent than the media containing unirradiated chitosan.