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International Journal of Recent Trends in Science and Technology, ISSN 2277-2812 E-ISSN: 2249-8109

 

Volume 3, Issue 1, 2012 pp 01-04

 

Short Communication

 

Radiation sensitivity of Listeria monocytogenes planktonic and biofilm-associated cells

T. Mahami1, A. Adu-Gyamfi1 and S.T.Odonkor2

1Biotechnology and Nuclear Agriculture Research Institute, Ghana Atomic Energy Commission, P.O. Box LG 80, Legon, GHANA.

2Nutrition Research Centre, Radiological and Medical Sciences Research Institute, Ghana Atomic Energy Commission, P.O. Box LG 80, Legon, GHANA.

Academic Editor:  Dr. Pawar D.D.

 

Abstract

Biofilms of Listeria monocytogenes were formed on sterile glass slides in a coincubation apparatus, using inoculated tryptic soy broth, incubated at 37C for 48 h. The planktonic and biofilm cultures were subjected to incremental gamma radiation doses (0, 0.5, 1.0, 1.5 , 2.0 kGy) from a Cobalt-60 source. The D10 values were calculated from the linear regression model for the logarithm of the surviving fraction and irradiation dose. The survivors and surviving fraction of planktonic and biofilm-associated cells decreased with increased irradiation doses. The D10 value of L. monocytogenes planktonic cells (0.476KGy) was higher than that of biofilm-associated cells (0.379KGy) indicating biofilm cells were more sensitive to ionizing radiation than planktonic cells. The antimicrobial efficacy of ionizing radiation is therefore preserved or enhanced in the treatment of biofilm-associated bacteria.