Hinge1, V. V. Patil1, R. L. Chavhan3,
and G. C. Jadeja2
Dept of Biotechnology, COCSIT, Latur-413512,
Dept of Agril Botany, AAU, Anand-388110, Gujarat,
Dept of Plant Biotechnology, COAB, Latur-413512.
Received 12 August 2011;
Revised 22 August 2011; Accepted 30 August 2011
Eighteen Root nodule bacterial isolates
were collected from the chickpea, grown in different areas of middle
Gujarat, viz Anand, Dahod, Thasara, Arnej, and Dhanduka.
Phenotypic and molecular characteristics of these chickpea Root nodule
bacteria (RNB) isolates were studied with eight other reference strains
of Rhizobium and one strain of Agrobacterium. The RNB
isolates showed diverse whole cell protein profile, which grouped them
according to their geographical origin. All the RNB isolates and
standard strains were clustered into three
major clusters. Eight RNB isolates were clustered in first group along
with six other standard strains at 57 % similarity. Second group
comprised remaining all RNB strains from Anand, Dahod, Dhanduka and
Thasara at 69 % similarity. Three standard strains of Rhizobium
along with one Agrobacterium strain grouped into third cluster.