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International Journal of Recent Trends in Science And Technology, E-ISSN: 2249-8109 

Volume 1, Issue 1, 2011 pp 01-05

Date of Publish: 30 Aug 2011

Research Article 

Whole Cell Protein Profile of Chickpea Root Nodule Bacteria Isolated From Different Areas Of Middle Gujarat. 

V. R. Hinge¹, V. V. Patil¹, R. L. Chavhan³, and G. C. Jadeja² 

¹ Dept of Biotechnology, COCSIT, Latur-413512,

² Dept of Agril Botany, AAU, Anand-388110, Gujarat,

³ Dept of Plant Biotechnology, COAB, Latur-413512.

Received 12 August 2011; Revised 22 August 2011; Accepted 30 August 2011

Academic Editor: Dr. Pawar D.D.

Eighteen Root nodule bacterial isolates were collected from the chickpea, grown in different areas of middle Gujarat, viz Anand, Dahod, Thasara, Arnej, and Dhanduka.  Phenotypic and molecular characteristics of these chickpea Root nodule bacteria (RNB) isolates were studied with eight other reference strains of Rhizobium and one strain of Agrobacterium. The RNB isolates showed diverse whole cell protein profile, which grouped them according to their geographical origin. All the RNB isolates and standard strains were clustered into three
major clusters.  Eight RNB isolates were clustered in first group along with six other standard strains at 57 % similarity. Second group comprised remaining all RNB strains from Anand, Dahod, Dhanduka and Thasara at 69 % similarity. Three standard strains of Rhizobium along with one Agrobacterium strain grouped into third cluster.