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International Journal of Recent Trends in Science and Technology, ISSN 2277-2812 E-ISSN: 2249-8109

Volume 10, Issue 2, March 2014 pp 378-385

Research Article

Behavioral and Histological Observations after the Human Amniotic Epithelial Cells Transplantation in the 6-Hydroxydopamine Induced Parkinsonism Disease Model in Wistar Albino Rats

Ravisankar Periyasamy*, Omprakash Kasaragod Venkatkrishnayya**, Muthusamy Rathinasamy***, Rameshkumar Radhakrishnan****, Ravindran Rajan*****, Sheeladevi Rathinasamy*****

*Associate Professor, Department of Anatomy, Tagore medical college and Hospital, Rathinamangalam, Vandalur Melakottaiyur Post, Chennai -600 127, Tamil Nadu, INDIA.

**Associate Professor, Department of Anatomy, Hassan Institute of Medical Sciences, Hassan, Karnataka INDIA

***Professor and Head, Department of Anatomy, S.R.M Dental College, Deemed University, Ramapuram, Chennai, Tamil Nadu, INDIA.

{****Assistant professors, Department of Anatomy} {*****Associate Professor, Department of Physiology} Dr. A. L. Mudaliar Post Graduate Institute of Basic. Medical Sciences, University of Madras, Taramani Campus, Taramani, Chennai -113, Tamil Nadu, INDIA.


Academic Editor : Dr. Aher K. R.


Several toxin-induced animals’ models simulate the motor deficits occurring in PD. Among them, the unilateral 6-hydroxydopamine (6-OHDA) model is frequently used in rats and has the advantage of presenting side-biased motor impairments. The studies on impairment and improvement of the motor and sensory motor behaviors after the retrograde degeneration with the HAE cells implantation are less or unavailable. In the present work we have studied the amelioration of motor and sensory motor behaviors after the implantation of human amniotic epithelial (HAE) cells in 6-hydroxydopamine (6-OHDA) lesioned rodent model of PD induced rats. The Sterotaxic injection of saline alone (without 6-OHDA) in the striatum could not produce any detectable effects on the behaviors and histological observations. This study demonstrates that substantial and stable improvement of behaviors after the HAE transplantation in unilateral 6-OHDA-induced lesions can be established in rodent model of PD disease, and that these canbe functionally assessed using several different behavioral tests. This would suggest that the HAE cells may be a suitable donor tissue to alleviate various degenerative diseases in animal model before the clinical trial in human who are suffering from the various degenerative diseases. The present study indicates that the transplantation of HAE cells could be a viable therapeutic strategy to slow down the progressive degeneration of striatal DA neurons in the animal model of PD. Thus the neurological degenerative disease can be treated by the grafting the HAE cells and can be attempted for faster and sustained recovery of functional loss in the human clinical trials.