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International Journal of Recent Trends in Science and Technology, ISSN 2277-2812 E-ISSN: 2249-8109

Volume 10, Issue 3, April 2014 pp 461-464

Research Article

Modified Petroff’s Method: an Excellent Simplified Decontamination Technique in Comparison with Petroff’s Method


Kiran Tripathi1, Purti C. Tripathi2, Shashwati Nema3, Arun Kumar Shrivastava4, Kalpana Dwiwedi5, Ashok Kumar Dhanvijay6

{1,3Associate Professor, 2Assistant Professor, 5Laboratory Technician, 6Professor and Head, Department of Microbiology}

{4Assistant Professor, Dept. of TB and Chest}

L. N. Medical College and Research Centre, Bhopal, Madhya Pradesh, INDIA.

Academic Editor : Dr. Aher K.R.



Tuberculosis is a major public health problem. In India, about 40% of the population is infected with TB bacillus. For diagnosis of TB, smear microscopy is still the most used due to its simplicity though it lacks sensitivity as compared with culture. This study compared the original Petroff’s method with Modified Petroff’s method. Spot and early morning sputum samples of 225 suspected tuberculosis cases were collected and processed by petroff’s and Modified Petroff’s method separately. Treated samples were cultured in Lowenstein-Jensen media. Out of 225 processed sputum specimens, 123 (54.67%) were smear positive by direct, 127 (56.44%) by Petroff’s method and 129 (57.33%) by Modified Petroff’s method. The number of culture positives by Modified Petroff’s method was 55.56%, by Petroff’s method were 53.33% and 45.33% by Direct method. Culture positives by Modified Petroff’s method compared with Direct method are statistically significant (P=0.0249, Chi square test). Negative culture and contamination rate was minimum with Modified Petroff’s method. Modified Petroff’s method requires lesser time, has lower contamination rate and higher yield in culture positivity as compared with Petroff’s method, making it a more suitable and better method of concentration and decontamination.