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International Journal of Recent Trends in Science and Technology, ISSN 2277-2812 E-ISSN: 2249-8109
Volume 11, Issue 3, July 2014 pp 313-315
Appraisal of oxidative stress markers and antioxidant status in diabetic neuropathy
Shilpashree Y. D.1, Devaki R. N.2, Suma M. N.3, Prashanth V.4
1Assistant Professor, Department of Biochemistry, Adichunchanagiri Institute of Medical Sciences, BG Nagara, Nagamangala,-571448, Karnataka, INDIA.
2Associate Professor, 3 Professor and HOD, 4Associate Professor, Department of Biochemistry, JSS Medical College, Mysore-570015, Karnataka, INDIA.
Introduction: Oxidative stress resulting from enhanced free-radical generation and/or a decrease in antioxidant defenses has been implicated in the pathogenesis of diabetic neuropathy. This study was conducted to evaluate oxidative stress and antioxidant balance in diabetic neuropathy and to correlate this with glycemic control. Method: Thirty patients with diabetic neuropathy and thirty age matched healthy controls were included in the study. Fasting blood glucose and glycosylated hemoglobin (HbA1C) were estimated to assess the severity of diabetes and the glycemic control respectively. Serum malondialdehyde (MDA) levels were assessed as a marker of lipid peroxidation and hence oxidative stress. Erythrocyte glutathione peroxidase levels were assessed for antioxidant status. Results: hba1c in patients with diabetic neuropathy compared to healthy control was statistically highly significant (p<0.000). Significant positive correlation was found between serum MDA levels and hba1c (r = 0.276, p < 0.0001) in patients with diabetic neuropathy. There was statistically significant reduction in the Glutathione peroxidase levels. Further, MDA levels were inversely correlated with GPx (r = - 0.70, p < 0.0001) levels. Conclusion and Summary: oxidative stress is greatly increased in patients suffering from diabetic neuropathy and is inversely related to glycemic control. This may be due to depressed antioxidant enzyme levels and may also be responsible for further depletion of antioxidant enzyme GPx. This worsens the oxidative stress and creates a vicious cycle of imbalance of free radical generation and deficit of antioxidant status in these patients which may lead to nervous system damage causing diabetic neuropathy. A good glycemic control is essential for prevention of diabetic neuropathy.