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International Journal of Recent Trends in Science and Technology, ISSN 2277-2812 E-ISSN: 2249-8109

Volume 11, Issue 3, July 2014 pp 383-385

Review Article

In vitro multiplication of Ceropegia bulbosa var bulbosa Roxb

Rohit Umesh Shete

Abstract

Ceropegia bulbosa var bulbosa Roxb is member of the family Asclepiadaceaewhich was described by William Roxburgh. It is very widely distributed, growing sporadically almost throughout India in red laterite soil. The leaves are subsessile to petiolate while Ceropegia bulbosa var. bulbosa has orbicular to ovate leaves. It cures the disorder Urolithiasis which is the development of stones in the urinary tract. This may lead to pain and bleeding. It is considered as the third most common affliction of the urinary tract. In most of the types of stones that are formed, the most frequent are calcium oxalate. As per clinical and epidemiological studies, calcium oxalate followed by calcium phosphate is the most commonly encountered crystalline components found in urolithiasis. Nodal explants from mature plant of this species were collected and cultured on MS medium supplemented with various concentrations (0.5, 1.0 and 3.0 mg l-1) of cytokinins (BAP and Kn) and auxins (IAA, NAA and 2, 4-D) alone and in various combinations under controlled condition. Successful regeneration method was achieved by this method.