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International Journal of Recent Trends in Science and Technology, ISSN 2277-2812 E-ISSN: 2249-8109

Volume 11, Issue 3, July 2014 pp 390-393

Research Article

A 5-year retrospective review on mycotic keratitis shows change in fungal trend at tertiary care hospital, Kishanganj, Bihar

Ghosh K¹,Ghosh B², Sarkar S³, Haldar N4, Ghosh A5, Mandal K6

1,3Assistant Professor, 4Professor, 5Intern, Kishanganj, Department of Microbiology and Ophthalmology, M G M Medical College and LSK Hospital, Kishanganj, Bihar, INDIA.

2Assistant Professor, ICARE Medical College, Haldia, Bihar, INDIA.

6Lab Technologist RSF Kolkata, West Bengal, INDIA.



Background: Monoocular blindness resulting from corneal ulcer due to my cotic keratitis is preventable in all developing countries like India. The early diagnosis by Ophthalmologist and microbiologist is the clue for prevention and recovery. Typical history of corneal injury with plant, use of antibiotics and steroid, injuries cleaning with pond water or stored pipe-line water are significant. The typical feathery ulcers with satellite lesions in one eye of the patients were selected for corneal scrape smear for KOH and Gram stain, fluorescence microscopy followed by fungal culture. Aims: To study the incidence and types of fungi involved. Materials and Methods: In the above cases corneal scraping was carried out for smear and culture examination under proparacaine local anesthesia. Scraping was done with no. 15 Bard Parker knife after removing loose debris with sterile cotton swab. It was immediately inoculated into Sabourauds dextrose agar and KOH mount was also done. Lactophenol cotton blue stain for fungus was used on mycelialisolates. Results: From May 2009 to April 2014, seventy eight (78) patients were diagnosed as Mycotic keratitis in our Medical College. Sixty-five cases were both culture and KOH preparation positive (fungal hyphae detected). Thirteen cases were only KOH preparation positive. Conclusion: From May 2009 to January 2012, the predominant fungi isolated in culture were Aspergillusspp., especially flavus. But since then the establishment of timed pipe-line water supply in this region most probably was responsible for the change in causative fungal predominance in culture as Fusarium Spp. Fusarium spp. are capable of producing biofilm in the pipe-line or in stored water container. Further research work is still in progress here in our Medical College.