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International Journal of Recent Trends in Science and Technology, ISSN 2277-2812 E-ISSN: 2249-8109
Volume 12, Issue 1, September 2014 pp 13-16
Inducible clindamycin resistance in Staphylococcus aureus isolatedfrom clinical samples
K. Vidyasagar1, R. Ravikumar2
1Assistant Professor, Department of Microbiology, Adichunchanagiri Institute of Medical Sciences BG Nagar, Taluka Nagamangala, Dist Mandya-PIN:571448, Karnataka, INDIA.
2Professor and HOD, Department of Neuromicrobiology, NIMHANS, Hosur Road, Bangalore-560029 Karnataka, INDIA.
Purpose: Clindamycin is commonly used in the treatment of erythromycin resistant Staphylococcus aureus causing skin and soft tissue infections. In vitro routine tests for clindamycin susceptibility may fail to detect inducible clindamycin resistance due to erm genes resulting in treatment failure, thus necessitating the need to detect such resistance by a simple D test on routine basis. Materials and Method: 100 Staphylococcus aureus isolates from various clinical samples subjected to routine antibiotic susceptibility testing by Kirby Bauer disc diffusion method. Inducible clindamycin resistance was detected by D test, as per CLSI guidelines on erythromycin resistant isolates. Results: 42 (42%) isolates showed inducible clindamycin resistance, 13 (13%) showed constitutive resistance while 10(10%) showed MS phenotype. Inducible resistance was found to be higher in Methicillin resistance Staphylococcus aureus (MRSA) as compared to Methicillin sensitive Staphylococcus aureus (MSSA) (56.6%, 13.2% and 25.53%, 12.7% respectively) Conclusion: Study showed that D test should be used as a mandatory method in routine disc diffusion testing to detect inducible clindamycin resistance.