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International Journal of Recent Trends in Science and Technology, ISSN 2277-2812 E-ISSN: 2249-8109

Volume 12, Issue 2, September 2014 pp 270-272

Research Article

Phenotypic characterization of coagulase negative staphylococci from various clinical isolates

Mamta Gour¹, Kalpana Date², V. R. Thombare³, K. K. Gour⁴

¹Assistant Professor, ⁴Associate Professor, Department of Anatomy, Department of Microbiology, L.N.M.C. Bhopal, Madhya Pradesh, INDIA.

Abstract

Background: Despite their frequency as contaminants, coagulase-negative staphylococci (CNS) have become important nosocomial pathogens, accounting for 9% of all nosocomial infections. These infections are difficult to treat because of the risk factors and the multiple drug resistance shown by these organisms. Material and Methods: One hundred and forty CNS were isolated from various clinical samples like blood, pus, urine body fluids, urine, catheter tip, gastric lavage and wound swab. After confirming the isolates as CNS, species level identification was performed by simple, nonexpensive conventional methods and antibiotic sensitivity testing was also carried out. Result: 140 isolates could be identified to species level. Among these 140 identified CNS isolates, S. saprophyticus was the most frequently isolated 58 (41.43%) from various clinical sample, followed by S. epidermidis 45(32.14%), S. lugdunensis 23(16.43%), S. hemolyticus 11(7.86%), S. schleiferi 3(2.14%). In the present study, the susceptibility pattern of CNS species against antimicrobial agents showed that 65.71 % of the isolates were Methicillin Sensitive (MSCNS). Majority of the CNS species were sensitive to Amikacin, (94.29%), Nitrofurantoin (85.71%), Tetracycline (75.71%), and clindamycin (75%). None of the CNS species showed resistance to vancomycin and Linezolid. Conclusion: The increased pathogenic potential and multiple-drug resistance demonstrates the need to adopt simple, reliable and non-expensive methods for identifying and determining the antibiotic sensitivity of CNS.