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International Journal of Recent Trends in Science and Technology, ISSN 2277-2812 E-ISSN: 2249-8109
Volume 12, Issue 2, September 2014 pp 239-242
Evaluation of Oxidative stress markers and antioxidant status in diabetic retinopathy
Shilpashree Y D1*, Suma M N2, Devaki R N3, Prashant V4
1Assistant Professor, Department of Biochemistry, Adichunchanagiri Institute of Medical Sciences, B G Nagara, Nagamangala, Karnataka-571448 INDIA.
2Professor and Head, 3Associate Professor, 4Associate Professor, Department of Biochemistry, JSS Medical College, JSS University, Mysore, Karnataka-570015 INDIA.
Introduction: Oxidative stress resulting from enhanced free-radical generation and/or a decrease in antioxidant defenses has been implicated in the pathogenesis of diabetic retinopathy. This study was conducted to evaluate oxidative stress and antioxidant balance in diabeticretinopathy and to correlate this with glycemic control Method: Thirty patients with diabetic retinopathy and thirty age matched healthy controls were included in the study. Fasting blood glucose and glycosylated hemoglobin (HbA1C) were estimated to assess the severity of diabetes and the glycemic control respectively. Serum malondialdehyde (MDA) levels were assessed as a marker of lipid peroxidation and hence oxidative stress. Erythrocyte glutathione peroxidase (GPx) levels were assessed for antioxidant status. Results: HbA1c in patients with diabetic retinopathy compared to healthy control was statistically highly significant (p<0.000). Significant positive correlation was found between serum MDA levels and HbA1c (r = 0.387, p < 0.0001) in patients with diabetic retinopathy. There was statistically significant reduction in the Glutathione peroxidase levels. Further, MDA levels were inversely correlated with GPx (r = - 0.90, p < 0.0001) levels. Conclusion and summary: oxidative stress is greatly increased in patients suffering from diabetic retinopathy and is inversely related to glycemic control. This may be due to depressed antioxidant enzyme levels and may also be responsible for further depletion of antioxidant enzyme GPx. This worsens the oxidative stress and creates a vicious cycle of imbalance of free radical generation and deficit of antioxidant status in these patients which may lead to nervous system damage causing diabetic retinopathy. A good glycemic control is essential for prevention of diabetic retinopathy.