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International Journal of Recent Trends in Science and Technology, ISSN 2277-2812 E-ISSN: 2249-8109
Volume 12, Issue 3, October 2014 pp 443-445
Detection of ESBL (extended spectrum β lactamases) from urinary isolates of multi drug resistant enterobacteriaceae in a tertiary care hospital in Mangalore
Lumbeni Kithan1, Thomas S Kuruvilla2, Tina Damodar1
1Post Graduate Resident, 2Associate Professor, Department of Microbiology, Father Muller Medical College, Mangalore, 575002, INDIA.
Introduction: The incidence of Extended Spectrum β Lactamase (ESBL) producing strains is increasing over the years. ESBL producing organisms pose problem for treatment. ESBL producers are also higher in uropathogens and baseline knowledge of ESBL organisms is mandatory for formulating control measures hence an attempt was therefore made to study ESBL production from urinary samples at a tertiary care hospital at Mangalore. Aim: To detect the number of ESBL producers among uropathogens in our centre and to compare two methods of ESBL detection. Materials and Methods: 1121 urinary samples were processed, of this 319 were Multi-drug resistant and were tested for ESBL production by Double Disc approximation and CLSI Confirmatory Test. Results: Out of 319 MDR samples, 153 were Enterobacteriaceae 93 were positive for ESBL i.e., 60.78% .Inpatients were more than outpatients and the two methods of ESBL detection were comparable. In this study ESBL was 60.78% among MDR enterobacteriacea and enterobacteriacea being the commonest organism in UTI, an attempt to contain ESBL organism may have some impact on decreasing the load of MDR organism. Conclusion: The burden of ESBL's among Enterobacteriacea uropathogens continues to pose a challenge in treatment. Carbapenems were a mainstay in treating these cases and either methods of ESBL detection may be conviently used. However attempts to minimize ESBL spread through hygiene,contact precautions,suitable antibiotic policy to include cheaper and more effective antimicrobials are the need of the hour.