[Abstract] [PDF] [HTML] [Linked
International Journal of Recent Trends in Science and Technology, ISSN 2277-2812 E-ISSN: 2249-8109
Volume 14, Issue 3, April 2015 pp 544-548
A study of relationship between leucocytospermia and specific seminal function parameters
Gajanan G Atram1, Pradnya G Atram2, S B Kaore3
1Professor, 2Associate Professor, Department of Physiology, Government Medical College ,Akola ,Maharashtra, INDIA.
3Professor, Department of Physiology, Government Medical College, Nagpur, Maharashtra, INDIA.
Introduction: Much controversy surrounds the clinical significance of an increased concentration of white blood cells (WBC) in the male ejaculate. The World Health Organization’s classification of leucocytospermia is a concentration >1x106 WBC/ml. Considerable evidence indicates that white blood cell concentrations are often elevated in semen of infertility patients and that WBC products can affect sperm function in vitro. Defective sperm function is the most common cause of infertility, and until recently, it was difficult to evaluate and treat. Part of this difficulty was due to our incomplete understanding of the factors contributing to normal and abnormal sperm function leading to male infertility. Aims and objectives: To study the relationship between leucocytospermia and specific seminal function parameters. Material and methods: The present study was conducted in the Semenology laboratory of department of Physiology, Government Medical College, Nagpur. The study involved 79 subjects with complaint of infertility referred from various Departments of Government Medical College and hospital, Nagpur. These subjects were grouped into three study groups according to leucocytes count in semen. While performing the semen analysis special emphasis was given on Hypo-osmotic swelling test, Acrosome intactness test, Nuclear chromatin decondesation test and sperm mitochondrial activity index.The subject included were in the age group of 21 to 40 yrs, they were all non smokers, non- alcoholics and free of any obvious genital tract abnormalities. The findings of seminal function were compared with the three groups of patients formed according to leucocytes count. Results: It was observed that, HOS score (%) was found to be more in case Group I (55.93±3.17) as compared to Group II and III (39.09 ±4.13 and 19.07±4.11 respectively). The Al test score (%) was lowest in group III (17.42±3.92) as compared to group I (54.68±3.79) and II (36.54 ± 3.46). Mean Nuclear Chromatin Decondensation Test score in group I was 55.03±3.70, in group II was 35.36 ± 2.93 and in group III was 18.14±3.35. Mean Sperm mitochondrial activity index was maximum in group I (56.20±3.08) followed by group II and III (35.90 ± 2.94 and 17.03±3.21respectively). When individual groups of leucocytospermia were compared with respect to the seminal function test score, difference in each group was statistically highly significant as compared to the other groups. Conclusion: Thus in the end we conclude that various seminal functional tests like Hypo-osmotic swelling test, Acrosome intactness test, Nuclear chromatin decondesation test and sperm mitochondrial activity index were affected by Leucocytospermia.