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International Journal of Recent Trends in Science and Technology, ISSN 2277-2812 E-ISSN: 2249-8109

Volume 15, Issue 1, May 2015 pp 62-64

Research Article

In vitro propagation of ayurvedic important plant Tinospora cordifolia (willd.) Miers

Megha Tupe1, Narayan Pandhure2

1,2Plant Tissue Culture Laboratory, Department of Botany, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad – 431001, Maharashtra, INDIA.

Tinospora cordifolia (Willd.) Miersis an important medicinal plant belongs to family Amaranthaceae found in India. The plant has medicinal properties like treat convalescence from severe illness, arthritis (or joint diseases), liver disease, eye diseases, urinary problems, anemia, cancer, diarrhea, and diabetes. It also helps to remove toxins from the body. The successful protocol for in vitro propagation has been achieved for the medicinal important plant T. cordifolia by using nodal and apical shoot tip segments as explants. In vitro plantlets raised on Murashige and Skoog (MS) medium containing 0.5–3.00 mg/l BAP in combination with 0.2-1.00 mg/l IAA, 3% sucrose, and 0.3% clerigar. After 21 days maximum percentage of shoot organogenesis was obtained on medium containing 2.0 mg/l BAP and 0.2 mg/l IAA. The regeneration protocol developed in this study provides an important method of micropropagation of this plant. Furthermore, this protocol may be used for a large scale production of its medicinally active compounds and genetic transformations for further improvement.