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International Journal of Recent Trends in Science and Technology, ISSN 2277-2812 E-ISSN: 2249-8109
Volume 15, Issue 2, June 2015 pp 245-250
In vitro nutrient composition of callus segments of Irvingia gabonensis (Aubrey-Lecomte Ex O’ Rorke, baill)
M M Etukudo1, O B Ilesanmi2, A Stephen3
1,2Department of Biological Sciences, Federal University Otuoke, P.M.B. 126, Yenagoa, Bayelsa State, NIGERIA.
3Department of Plant Science and Biotechnology, University of Port Harcourt, River State, NIGERIA.
Nutrient composition of callus segments of Irvingia gabonensis (Aubrey-Lecomte Ex O’Rorke, Baill) was studied using in vitro techniques. Disinfected explants were aseptically placed and inoculated on Murashige and Skoog (MS) callus induction medium (full -MS, half- ½ MS, and one-quarter-¼ MS, strengths) supplemented with plant growth regulators (PGR) with each treatment containing the following: control - states the absence of plant growth regulator (Mo), 0.1 mg/l BAP + 2.0 mg/l IBA (M1), 0.2 mg/l BAP + 4.0 mg/l IBA (M2), 0.3 mg/l BAP + 6.0 mg/l IBA (M3), 0.4 mg/l BAP + 8.0 mg/l IBA (M4), 0.5 mg/l BAP + 10.0 mg/l IBA (M5). Non- regenerative callus segments were formed in contamination-free apical bud cultures of I. gabonensis. Callus formation was evident after a three week period in culture for 1- 5 mg/l BAP + IBA of MS, ½ MS, and ¼ MS, and responses varied with media formulation. The medium giving the highest nutrient contents in each case was obtained from one-quarter strength growth medium followed by half strength, and full strength giving the least response. The control treatment (0 mg/l) of MS, ½ MS, and ¼ MS did not support any callus formation. Nutrient contents (potassium, calcium, phosphorus, nitrogen, magnesium, manganese, copper, zinc, iron and boron) in callus segments of I. gabonensis significantly (P < 0.05) increased with increase in hormone concentration, and with decrease in media strength. There were significant (P < 0.05) increases in soluble protein and carbohydrate contents in callus segments of I. gabonensis with increase in concentration of plant growth regulators, and with decrease in the concentration of growth medium. Therefore, this research has further unfolded the chemical and economic potentials of Irvingia gabonensis for continuous domestication of the species.