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International Journal of Recent Trends in Science and Technology, ISSN 2277-2812 E-ISSN: 2249-8109

Volume 15, Issue 3, July 2015 pp 524-525

Research Article

Comparison of auramine-O stain with Ziehl Neelson stain in suspected cases of tubercular lymphadenitis

Salman Akther1, Hilda Fernandes2, Gautam Goyal3

1,3PG Student, 2Professor and HOD, Department of Pathology, Father Muller Medical College, Mangalore, Karnataka, INDIA.

The objectives were to correlate the fluorescent method with the conventional Ziehl–Neelsen (ZN) method for the detection of acid-fast bacilli (AFB) and, also to study the efficacy and advantages of using the auramine–O stain on lymph node aspirates under fluorescent microscopy. 42 patients with a clinical suspicion of tuberculosis (TB) presenting with lymphadenopathy, fine needle aspirations were performed. Smears from the aspirates were processed for routine cytology, the conventional ZN method, and the fluorescent method. Out of 42 aspirates, The smear positivity by ZN was 26.2% (11/42) while positivity increases to 64.2% (27/42) cases with FT. Combined smear positivity with both the stains was 69.2% (29 /42) cases. Fluorescent microscopy has the advantage of speed and ease of screening, and reduces observer fatigue. The fluorescent method was found to be more advantageous than routine cytology and conventional ZN method, particularly in paucibacillary cases. The bacillary positivity rates were higher in the modified fluorescent method than in the ZN method.