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International Journal of Recent Trends in Science and Technology, ISSN 2277-2812 E-ISSN: 2249-8109

Volume 15, Issue 3, July 2015 pp 557-560

Research Article

In vitro multiplication of Podocarpus macrophyllus D. Don var. maki

Shewale M N1, Pandhure N B2

1,2Plant tissue Culture Laboratory, Department of Botany, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad, Maharashtra, INDIA.

Podocarpus macrophyllus var. maki is evergreen shrub or tree usually. It is drought tolerant; used as specimen, hedge or screen; 'Maki' is a slower growing version of the species, reaching only 20-35 ft. in height, 3-5 ft. in width. Some species of Podocarpus are used in systems of traditional medicine for conditions such as fevers, coughs, arthritis, sexually transmitted diseases, and canine distemper. Recently new compounds have been extracted from the leaves of Podocarpus. An efficient protocol for multiplication has been developed for this medicinal plant. Regeneration was achieved from nodal explants raised on Murashige and Skoog medium (MS) containing BAP (0.5-3.0mg/l), 3% sucrose and 2.8 gm/l Clarigar along with IAA (0.2mg/l). The regeneration protocol developed during this piece of work provides an important method for micropropagation of this plant in vitro.