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International Journal of Recent Trends in Science and Technology, ISSN 2277-2812 E-ISSN: 2249-8109

Volume 15, Issue 3, July 2015 pp 463-472

Research Article

Isolation of Withaferin-A from Withania Somnifera plant root and its effects on cancer rats

S Murugan1, M Ameesh2, G Ekambaram3, R Devaraja4, R Sundaram5, V Ashok6, S Shilpa7,  D Sakthisekaran8

1,6Assistant Professor, 2,7Tutor, Government Medical College, Palakkad, Kerala, INDIA.

3Assistant Project Scientist, California University, USA.

4,5Post-doctoral fellow, Virginia Commonwealth University, USA.

8Professor, Dr. ALM PGIBMS University of Madras, Taramani, Chennai- 600113, Tamil Nadu, INDIA.

Background: Hepatocellular carcinoma (HCC) is the most universal primary malignant tumor of the liver. It is the third most common cause of cancer related deaths in men and the ninth in women caused by chemical carcinogens. Chemotherapy is a systemic treatment, which works throughout the body to kill cancer cells. Aim and Objectives: The purpose of this study is to investigate the oxidative biomarkers and hepatoprotective efficacy of Withaferin-A (WFA) against hepatocellular carcinoma in experimental rats. Materials and Method: Group I - Control animals treated with 0.5% of Carboxymethyl cellulose (CMC) orally throughout the experimental period. Group II- Hepatocellular carcinoma was induced by providing 0.01% DEN through drinking water for 15 weeks. Group III - Animals were treated with Withaferin-A at the dosage of 50mg/kg body.wt, orally for 21 days before administration of DEN as in Group II. Group IV - Hepatocellular carcinoma induced animals treated with Withaferin-A at a dosage similar to group III for 21 days, i.e. after the administration of DEN for 12 weeks. Group V -Animals treated with Withaferin-A (as in Group III) alone for 21 days. Results: In this study, we observed that WFA protects the rats from DEN induced HCC when administered at a lethal dose of 50 mg/kg for 21 days. The levels of lipid peroxidation, protein carbonyls and liver marker enzymes were markedly increased in cancer bearing animals. In contrast, the activities of the enzymic and Non-enzymic antioxidants were found to be decreased in liver of cancer bearing animals. The cancer bearing animals when treated with Withaferin-A showed significantly decreased levels of liver marker enzymes with simultaneous reversion in the activities of antioxidants levels when compared to the cancer induced animals. Conclusion: These observations suggest that the treatment with WFA effectively reduced oxidative stress, as assessed by decreased levels of various oxidants and improved the level of diverse antioxidants. The obtained results it is concluded that withaferin-A is capable of restoring the liver architecture by increasing the antioxidant status in hepatocarcinogenic rats.