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International Journal of Recent Trends in Science and Technology, ISSN 2277-2812 E-ISSN: 2249-8109

Volume 16, Issue 1, Aug 2015 pp 74-78

Research Article

Study of gram negative septicemia in cases of neonates admitted in neonatal intensive care unit at tertiary care hospital

Preeti M Huggi1, Sanjeev B Navadagi2

1,2Ex Resident, Department of Microbiology, M R Medical College, Kalaburagi, Karnataka, INDIA.

Background: Septicemia in neonates is one of the four leading causes of neonatal mortality in India. The pattern of causative agents has been constantly changing and there has been a frequent emergence of resistant bacteria. The antibiotic misuse has resulted in emergence of drug resistant bacterial strains in the neonatal units with grave sequelae. Objectives: to describe the spectrum of aerobic gram negative bacterial isolates and study the sensitivity pattern in cases of neonatal septicemia. Methods: Blood samples were collected aseptically from 200 clinically suspected cases and inoculated into BHI broth and incubated at 370 C for 7 days. Repeated subcultures were made on 1st, 3rd and 5th days onto Blood agar and Mac Conkey agar. Any growth was identified by colony characteristics and appropriate biochemical tests. Antibiotic susceptibility was done by disc diffusion method as per CLSI guidelines. Results: Of the 200 cases, 31.5% were found to be culture positive. Gram negative isolates were predominant 73% and Gram positive isolates were obtained in 27% of the cases. Out of the Gram negative, Klebsiellaspp was the commonest (44.4%) followed by Pseudomonas aeruginosa (17.5%), Escherichia coli (9.5%), Proteus vulgaris (1.6%). Gram negative isolates were most sensitive to Imipenem and Meropenem (98%), followed by Cefoperazone /Sulbactum (75-80%), Amikacin and Gentamycin (70-75%) and least sensitive to third generation cephalosporins. Conclusion: Therefore uncertainty regarding the choice of antibiotics can be minimized by regular survey of etiological agents and their antimicrobial susceptibility pattern.