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International Journal of Recent Trends in Science and Technology, ISSN 2277-2812 E-ISSN: 2249-8109

Volume 16, Issue 1, Aug 2015 pp 96-100

Research Article

MRSA and MRSE: Its prevalence, antibiotic sensitivity and correlation with biofilm formation observed in various clinical isolates

Deepika Atray1, Anshu Sharma2, Meena Atray3

1MSc Medical Microbiology, 2,3Associate Professor, Department of Microbiology, RNT Medical College, Udaipur-313001, Rajasthan, INDIA.

Abstract
Biofilm formation has become a major factor in enhancing the antibiotic resistance of staphylococci. In the present study, we observed methicillin resistance and further biofilm production by two methods – Congo red agar method and Christenson’s test tube method and compared the antibiotic resistance pattern between biofilm producers and non biofilm producers. 102 S. aureus and 168 S. epidermidis were isolated from various clinical samples with 70.5% and 79.7% methicillin resistance resp.51.4% MRSA and 61.2% MRSE were biofilm producers. Antibiotic sensitivity was observed in biofilm producing S. epidermidis and S aureus for Vancomycin (63%/85%), Doxycycline (48%/61%), Tetracycline (41%/52%), Cephalexin (19%/15%), Cefotaxime (41%/43%), Ceftrioxone (31%/37%), Cotrimoxazole (10%/11%), Ciprofloxacin (45%/52%), Amoxicillin (34%/35%), Ampicillin (4%/2%), Amoxyclave (26%/17%), Erythromycin (6%/14%), Amikacin (71%/76%), Gentamycin (66%/65%) and for urinary isolates Norfloxacin (9%/11%) and Nitrofuranoin (57%/78%) which was significantly less as compared to non biofilm producers.