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International Journal of Recent Trends in Science and Technology, ISSN 2277-2812 E-ISSN: 2249-8109

Volume 16, Issue 2, Sept 2015 pp 253-257

Research Article

Impression cytology in the diagnosis of ocular surface squamous neoplasia (OSNN)

Tulasi Priya Kandyala1, K Dayasagar Reddy2, P Modini3, T Kavitha4

1Assistant Professor, 2Associate Professor, Department of Ophthalmology, Bhaskar Medical College, Hyderabad, Andhra Pradesh, INDIA.

3,4Assistant Professor, Department of Pathology, Osmania Medical College, Hyderabad, Andhra Pradesh, INDIA.

Introduction: Ocular Surface Squamous Neoplasia (OSSN) describes a spectrum of conjunctival and corneal epithelial neoplasia manifesting as dysplasia, Carcinoma-In-Situ (CIS) and Squamous Cell Carcinoma (SCC). Impression cytology refers to the application of cellulose acetate filter to the ocular surface to remove superficial layers of the ocular surface epithelium. The cells thus removed are subjected to haematoxylin and eosin staining. Objectives: To evaluate the accuracy of impression cytology in the diagnosis of Ocular Surface Squamous Neoplasia by comparing it with the histopathology of the excised specimens. Methods: It was a prospective, interventional case series conducted from December 2007 to June 2009 at Sarojini Devi Eye Hospital. 50 eyes of 48 patients with clinical suspicion of OSSN were included. Impression cytology was done using biopore membrane and reported by a single Pathologist, following which excision biopsy was performed. Cytology and histopathology reports were compared and correlation was said to be present if dysplastic cells were present on cytology in a patient found to have neoplasia on histopathology. Results: Out of 50 eyes with clinical suspicion of OSSN, 44 eyes were found to have neoplasia on histopathology. Correlation between impression cytology and histopathology was present in 38 eyes (86.36%). Conclusion: Impression cytology is a simple, non-invasive technique with a high correlation rate in the diagnosis of OSSN. It is especially useful in patients with suspected recurrence. Presence of hyperkeratosis on cytology has a poor correlation with histopathology.