Abstract Introduction: Nodal metastasis remains the single most important prognostic factor for patients with penile cancer. Over half of patients with penile squamous cell carcinoma (SCC) have clinically impalpable inguinal nodes at presentation, and up to 20% of these will harbor clinically occult micro metastases (< 2 mm). Clinical examination and current imaging methods remain inaccurate for detecting micro metastases. Methodology: After approval from institutional ethical committee a prospective study on 30 patients who had penile cancer was carried out at tertiary health center conducted between Jan 2010 to Jan 2015. All the patients, after signature of informed consent, had undergone partial penectomy, biopsy of the sentinel lymph node from the negative inguinal regions and modified radical lymphadenectomy as proposed by Catalona (1988) in these regions regard­less of the biopsy results. Biopsy of the sentinel lymph node and classi­cal radical lymphadenectomy were performed in the positive inguinal regions Result: Total Malignancy detected by the Sentinel Lymph node biopsy were in 19 cases out of that 18 were confirmed by the Modified inguinal Lymphadenectomy 1 was falsely diagnosed as Malignant and in 11 malignancy negative sites 9 were true negative but in 2 cases malignancy was detected by Modified inguinal Lymphadenectomy so in this case Sensitivity of the Sentinel Lymph node biopsy = 90%, Specificity = 76.78%, Positive Predictive Value= 94.73%, Negative Predictive Value= 81.81%. Conclusion: Sentinel Lymph node biopsyprocedure is having high diagnostic reliability and accuracy as comparable with the gold standard procedures so is having valuable role in management of the penile cancer.

Keywords: Sentinel Lymph node biopsy, modified inguinal Lymphadenectomy, Penile Cancer.