Abstract  Introduction: Urinary tract infections (UTIs) remain the commonest infections diagnosed in outpatient as well as hospitalized patients. It refers to the presence of microbial pathogens within the urinary tract and classified by the site of infection - bladder [cystitis], kidney [pyelonephritis] or urine [bacteriuria] Aims and Objectives: To study of efficacy of purlifloxacin with other quinolons and fluroquinolones among the Escherchia coli isolates from various clinical specimens. Materials and Methods: A cross sectional study was conducted between November 2012 and March 2013 at Clinical Microbiology laboratory of Sri Ramachandra University, a tertiary care center with more than 1,700 in-patients facility where in the anti microbial susceptibility of various quinolones like Nalidixic acid, Norfloxacin, Ciprofloxacin, Ofloxacin and Prulifloxacin were determined for Escherichia coli isolated from urine by Kirby-Bauer disc diffusion method and compared with that isolated from blood and exudates. Non repetitive E.coli isolates from Urine, Exudate and Blood specimen meant for culture and sensitivity were included in the study. Total number of isolates was 100, in which 50 were obtained from urine, 25 from blood and exudates each were enrolled for the study. Antimicrobial Susceptibility Testing Done By Diffusion Method: The Modified Kirby-Bauer Method Was Used. Result: Out of the 100 samples tested 66 of them were male and 44 of them females. Most of the patients of our study belong to the age group of 60 and above with 15,13,6 patients respectively for urine, blood and exudates. The antimicrobial susceptibility pattern of E.coli isolates obtained from urine samples were the susceptibility for Ofloxacin were 25 (50%;n=50) followed by Prulifloxacin 17 (34%;n=50), Ciprofloxacin 18 (36% n=50) Norfloxacin 16 (32%;n=50), Nalidixic acid 8 (16%n=50) . Of the 25 blood E.coli isolates tested, 22 (88%; n=25) of them were susceptible to Ofloxacin, 19 (76%; n=25) susceptible to Prulifloxacin, 12 (48%; n=25) susceptible to Norfloxacin, 7(28%; n=25) susceptible to Ciprofloxacin and 3(12%;n=25) susceptible to Nalidixic acid. Among the 25 Exudate E.coli isolates 72% (18; n=25) were susceptible to Ofloxacin. The susceptibility pattern for Prulifloxacin was 34% (17; n=25).The susceptibility pattern of Ciprofloxacin, Norfloxacin and Nalidixic acid are 18% (9; n=25, 6% (3; n=25) and 2% (1; n=25) respectively. Overall susceptibility pattern for isolates obtained from Urine, Blood, Exudates were shown in the fig. , Ofloxacin 65%, and Prulifloxacin 55%, Ciprofloxacin 54%, Norfloxacin 35%, Nalidixic acid 12%. Conclusion: The study shows quinolones is a good drug for treating urinary tract infection. Ofloxacin is better drug than Prulifloxacin

Key Words: Purlifloxacin, Quinolons, Fluroquinolones,Escherchia coli isolates.