Abstract     Introduction: Type 2 Diabetes Mellitus is the commonest endocrinal disorder worldwide. A positive family history of Diabetes Mellitus increases the risk of developing the disease to manifold in off-springs. Serum fructosamine (glycated albumin) depicts short term glycaemic control. The purpose of the study is to estimate serum fructosamine and its comparison with the established markers in the non diabetic first degree relatives of type 2 Diabetes Mellitus. Aim and Objectives: 1) To measure waist circumference (WC), body mass index (BMI), fasting plasma glucose (FPG) and serum fructosamine in normal controls and non-diabetic first degree relatives of type 2 Diabetes Mellitus patients. 2) To compare WC, BMI, FPG and serum fructosamine in these groups. Material and Methods: The study was conducted in Dr. S.C. Govt. Medical College, Nanded from 22/1/2015 to 1/3/2015. Total 50 participants were selected and grouped as Group 1 consisting of 25 healthy controls and Group 2 consisting 25 non-diabetic first degree relatives of type 2 Diabetes Mellitus patients. WC, BMI, FPG and serum fructosamine were estimated and compared. The data was analyzed statistically. Results: In group 1 the mean values of WC, FPG and serum fructosamine were 68.9 ± 8.6 cm, 72 ± 9.5mg/dl and 211 ± 3.2 mg/dl respectively. In group 2 the mean values of WC, FPG and serum fructosamine were 86.5 cm, 104±6.7 mg/dl and 275 ± 3.2 mg/dl respectively which were significantly high as compared to group 1 .The correlation of serum fructosamine with WC and FPG in group 2 is positively significant as per Pearson’s correlation co-efficient. Conclusion: WC, FPG and serum fructosamine are elevated in first degree relatives of type 2 Diabetes Mellitus indicating that these patients have a high risk of development of Diabetes Mellitus. Serum fructosamine can be used as a risk assessor of type 2 Diabetes Mellitus but further studies on large scale are required.