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International Journal of Recent Trends in Science and Technology, ISSN 2277-2812 E-ISSN: 2249-8109

Volume 6, Issue 3, March 2013 pp 139-143

Research Article

Prevalence of Multidrug Resistance, ESBL and MBL production in Acinetobacter spp.


Purti Tripathi1, Sunita Gajbhiye2

1Assistant Professor, Department of Microbiology, LN Medical College, Bhopal, Madhya Pradesh, INDIA.

2Assistant Professor, Department of Microbiology, Indira Gandhi Govt. Medical College, Nagpur, Maharashtra, INDIA.


Academic Editor : Dr. Aher K.R.



Background: Acinetobacter are important cause of nosocomial infections with widespread resistance to various antibiotics. Extended spectrum beta lactamase (ESBL) and metallobeta-lactamase (MBL) associated resistance among Acinetobacter is now known. This study aims to determine antibiotic susceptibility patterns of Acinetobacter isolates, prevalence of multidrug resistance, ESBL production and MBL production. Material and methods: 107 Acinetobacter isolates were identified by standard microbiological testing. Antimicrobial susceptibility testing was performed by modified Kirby Bauer method as per the CLSI guidelines. Multidrug resistance was determined. ESBL production was detected by double disc method and CLSI phenotypic confirmatory test. MBL production was detected by combination disc test using imipenem and imipenem/EDTA disc. Results: The maximum sensitivity of Acinetobacter was seen to imipenem (57.00%) and amikacin (55.14%). Maximum resistance was observed to ceftazidime (100%), cefotaxime (100%) and piperacillin (100%). A. baumannii was more resistant to majority of drugs used and A. junii was more susceptible to majority of the drugs used. 32 (29.90%) Acinetobacter strains were extended spectrum beta lactamase (ESBL) producers. Out of the total 46 imipenem resistant Acinetobacter isolates, 40 (86.95%) Acinetobacter were MBL producer and all of them were A. baumannii strains. Multiple drug resistance was common among Acinetobacter isolates. Significantly higher percentage of multidrug resistance was found in A. baumannii strains compared to other Acinetobacter spp (P<0.05). Conclusion: Multidrug resistance in A. baumannii was more common compared to other spp. ESBL and MBL production should be promptly detected and reported to control the spread of resistant phenotypes to other individuals.