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International Journal of Recent Trends in Science and Technology, ISSN 2277-2812 E-ISSN: 2249-8109

Volume 9, Issue 2, December 2013 pp 275-280

Research Article

Human Amniotic Epithelial (HAE) Cell Grafts Establish Connections with Host Hippocampal Cells and Suppress Aberrant Host Mossy Fiber Sprouting of the Dentate Supragranular Layer in a Rat Model After Trimethyltin Chloride (TMT) Lesion


Kasaragod Venkatakrishnayya Omprakash1, Kanthaiah2, Rathinasamy Muthusamy3, Ravisankar Periyasamy4, Nagaraja Puranik5, Radhakrishnan Ramesh Kumar6, Matada Gurushanthaiah7

1Associate Professor, Department of Anatomy, Hassan Institute of Medical Sciences, Hassan, Karnataka, INDIA.

2Associate Professor, Department of Biochemistry, Hassan Institute of Medical Sciences, Hassan, Karnataka, INDIA.

3Professor, Department of Anatomy, Basaveshwara Medical College and Hospital, Chitradurga, Karnataka, INDIA

4Associate Professor, Department of Anatomy, Tagore Medical College & Hospital, Rathinamangalam, Chennai, INDIA.

5Associate Professor, Department of Physiology, Karnataka Institute of Medical Sciences, Hubli, Karnataka, INDIA.

6Assistant Professor, Department of Anatomy, Dr. ALM. PG. Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai, Tamilnadu, INDIA.

7Professor, Department of Anatomy, S.R.M. Dental College, Deemed University, Ramapuram, Chennai, INDIA.

Academic Editor : Dr. Aher K.R.



Introduction: The main aim of present study is to provide adequate numbers of cells to appropriate sites for useful cellular replacement to overcome the functional deficits caused by the trimethyltin chloride lesion. Methods: This study was conducted on the Wistar albino rats. Hippocampal disorder was induced by the intraperitoneal administration of trimethyltin chloride at single dose of 7.5 mg/kg body weight or two divided doses of 3.75 mg/kg body weight for two days. The human amniotic epithelial cells to be transplanted were isolated from the fetal surface of the human placenta obtained from uncomplicated elective caesarian. Using standard co-ordinates human amniotic epithelial cells were injected at four sites of hippocampal formation. For histological confirmations of the lesion and the growth of the transplant in  the hippocampus, rat brains were processed and stained after various post operative periods of  7, 15, 30, 60,120 and 150 days. Results:The human amniotic epithelial cells become converted into neuron like cells and establish  connections with host hippocampal cells and suppress aberrant host mossy fiber sprouting of the dentate supragranular layer. Conclusion: The present study concludes that the human amniotic epithelial cells may be used as a suitable donor tissue to alleviate various degenerative diseases in animal model before the clinical trial in humans, who are suffering from various degenerative diseases.