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International Journal of Recent Trends in Science and Technology, ISSN 2277-2812 E-ISSN: 2249-8109

Volume 9, Issue 2, December 2013 pp 178-181

Research Article

Isolation and Characterization of Hydrocarbon Degrading Bacteria’s isolated from Diesel Polluted Soil from Various Petrol-Diesel Bunk of Solapur


P. Mhamane1, Neelam Shaikh2, Sohani H.M.3, Rajashree4

1,2B.Sc. Student, 3Former faculty, Department of Biotechnology (WCBT)

Walchand College of Arts and Science, Solapur – 413006 Maharashtra, INDIA.

4B.Sc. Student, Department of Microbiology, Maharani Lakshmi Ammani College, Bangalore – 560012, Karanataka, INDIA.

Academic Editor : Dr. Aher K.R.



Research work deals with two aspects, firstly collection of diesel polluted soil samples from various petrol-diesel bunks from Solapur, secondly it deals with the laboratory work which includes culturing and sub-culturing to get pure culture of micro-organisms from soil sample, and study regarding characterization and degradation of hydrocarbons. Gram staining, sugar fermentation, biochemical and IMVIC test were carried out on isolated micro-organism which revealed the isolated micro-organisms were from Bravibacterium spp., Streptococcus spp., Bacillus spp., Enterobacter spp. Research work indicates that these isolates were capable to degrade hydrocarbons like diesel, anthracene, naphthalene and benzene.