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International Journal of Recent Trends in Science and Technology, ISSN 2277-2812 E-ISSN: 2249-8109

Volume 9, Issue 2, December 2013 pp 186-188

Research Article

Evaluation of Analgesic Activity of Aqueous Extract of Fenugreek Seeds in Albino Mice


Satish A. M.1, Brahadeesh M.2, Kalabharathi H. L.3, Pushpa V. H.4, Jayanthi M. K.5 Suresha R. N.6

1Associate Professor, 2Post Graduate Student, 3,4Associate Professor, 5,6Professor, Department of Pharmacology

JSS Medical College (A Constituent College of JSS University, Mysore) SS Nagar, Mysore, Karnataka, INDIA.

Academic Editor : Dr. Aher K.R.



Objective: To evaluate the analgesic activity of aqueous extract of fenugreek seeds on chemical and mechanical painful stimulus in albino mice. Methods: Albino mice (20-25g) were divided into three groups of 6 animal each as control -1st group, standard-2nd group and test-3rd group.1stgroup was Control and it received Gum acacia (2 g in 100 ml of distilled water), 2nd group was Standard and it received (indomethacin 10 mg/kg),3rd group was test group and it received aqueous extract of fenugreek seeds 200 mg/kg orally in the below mentioned methods. Acetic acid Induced Writhing Test (Chemical Stimulation). The writhing movements were observed and counted for 10 min after intraperitoneal administration of 0.2ml of 1%acetic acid. Tail clip method (mechanical stimulus). An artery clip is applied to the root of the tail to induce pain. The animal quickly responded by biting the clip or the tail. The time between stimulation onset and response is measured and the method repeated after 15 mins and 30 mins. Results: In acetic acid induced writhing test mean value of number of writhes is 46.8 in control group, 11.8 in standard group, 19 in test group and the percentage of inhibition is 0%, 75% and 60% respectively. In tail clip method at 0 min the mean value of reaction time in control group was 1.16secs, in standard group was 7.63 secs and in test group it was 4.74 secs. At 15 mins the mean value of reaction time in control group was 1.19 secs, in standard group was 8.67secs and in test groups it was 6.7secs. At 30 mins the mean value of reaction time in control group was 1.24 secs in standard group 9.62secs and in test group it was 7.10secs .Statistical analysis done by ANOVA in both the methods and p value  obtained was <0.001  and was statistically significant. Conclusion: Based on the results aqueous extract of fenugreek seeds exhibited analgesic activity.