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International Journal of Recent Trends in Science and Technology, ISSN 2277-2812 E-ISSN: 2249-8109

Volume 9, Issue 3, January 2014 pp 370-377

Research Article

Study of Biofilm Producing Property and Predisposing Factors in Bacterial Isolates from Community Acquired Urinary Tract Infections

Vishwajeet Bardoloi¹, Yogeesha Babu K.V.², Basavarajappa K.G.³

{¹Post Graduate Student, ³Professor and Head, Department of Microbiology}

SS Institute of Medical Sciences and Research Centre, Jnanashankara, NH-4 Bypass road, Davangere, INDIA.

Academic Editor : Dr. Aher K. R.

Introduction: Bacteria with biofilm producing property[BFPP] are associated with chronic and intractable urinary tract infections(UTI),especially with indwelling urinary catheter. Limited data on bacteria with BFPP in community acquired UTI[c-UTI] necessitated the present study. Aims and Objectives: To determine prevalence of biofilm producing property, predisposing risk factors and multi-drug resistance in bacterial pathogens from c-UTI. Material and Methods: Mid-stream urine specimens from75 patients, attending different OPDs were collected and processed by standard laboratory procedures. Bacterial isolates were tested for BFPP by Tube and Congo red dye tests. Predisposing risk factors and multi-drug resistance by interim guidelines incorporating CDC, EUCAST and FDA criteria were determined. Statistical analysis was done by Fisher’s exact test. Results: Prevalence of bacterial pathogens with BFPP from c-UTI was 83.75% (67/80). BFPP by Tube method alone, Congo red dye method alone and by both methods was 26.87% (18/67), 28.36% (19/67) and 44.78% (30/67) respectively. Prevalence of BFPP in E.coli, Klebsiella spp., Pseudomonas aeruginosa, Staphylococcus aureus and CONS was 96.43% (27/28), 78.95% (15/19), 85.71% (6/7), 77.78%(14/18) and 66.67%(4/6) respectively. Age, sex, department wise distribution, resistance for individual antibiotics and multi-drug resistance [53.75% (43/80) vs. 8.75%(7/80)] among bacteria with BFPP and non-BFPP was not statistically significant. Association of predisposing risk factors among isolates with BFPP was observed in only 24.61% (16/65) patients. Conclusions: Tube test and Congo red agar tests should be used together as screening tests to detect BFPP in bacterial isolates from c-UTI. BFPP was observed as natural property of bacteria rather than a virulence factor. No significant difference in multi-drug resistance observed among bacteria with BFPP and non-BFPP. Predisposing risk factors do not play role in acquisition or expression of BFPP in bacterial isolates from c-UTI.